Search result for 'flower '.
Viewing records 231 to 240 of 1605 hits.
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N16243
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Name: embryonic flower 2 |
Price:
£11.00
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Donor
- University of California, Berkeley Brian Staskawicz
- University of California, Berkeley Z. Renee Sung
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Locus
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Stock type: individual line
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Material type: seed
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Description
recessive
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Phenotype
Seedlings with short hypocotyls, oval-shape cotyledons with no petioles, absence of rosette growth, a very short inflorescence develops directly from the embryo or callus; the inflorescence shoot contains sessile leaves and 2 to 3 flowers with incomplete floral organ development; although all the four floral organs can be observed in a population, petal formation is devoid in most individuals; a pistil is the most prominent floral organ observed; homozygous plants are sterile.
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N16244
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Name: embryonic flower 2 |
Price:
£11.00
|
Donor
- University of California, Berkeley Brian Staskawicz
- University of California, Berkeley Z. Renee Sung
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Locus
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Stock type: individual line
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Material type: seed
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Description
recessive
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Phenotype
Seedlings with short hypocotyls, oval-shape cotyledons with no petioles, absence of rosette growth, a short inflorescence develops directly from the embryo or callus; the inflorescence shoot contains sessile leaves and 2 to 3 flowers with incomplete floral organ development; although all the four floral organs can be observed in a population, petal formation is devoid in most individuals; a pistil is the most prominent floral organ observed; homozygous plants are sterile.
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N16245
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Name: embryonic flower 2 |
Price:
£11.00
|
Donor
- University of California, Berkeley Brian Staskawicz
- University of California, Berkeley Z. Renee Sung
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Locus
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Stock type: individual line
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Material type: seed
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Description
Based on seeding-lethal screening
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Phenotype
Seedlings with short hypocotyls, oval-shape cotyledons with no petioles, absence of rosette growth, a short inflorescence develops directly from the embryo or callus; the inflorescence shoot contains sessile leaves and 2 to 3 flowers with incomplete floral organ development; although all the four floral organs can be observed in a population, petal formation is devoid in most individuals; a pistil is the most prominent floral organ observed; homozygous plants are sterile.
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N16246
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Name: embryonic flower 2 |
Price:
£11.00
|
Donor
- University of California, Berkeley Brian Staskawicz
- University of California, Berkeley Z. Renee Sung
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
recessive
|
Phenotype
Seedlings with short hypocotyls, oval-shape cotyledons with no petioles, absence of rosette growth, a short inflorescence develops directly from the embryo or callus; the inflorescence shoot contains sessile leaves and 2 to 3 flowers with incomplete floral organ development; although all the four floral organs can be observed in a population, petal formation is devoid in most individuals; a pistil is the most prominent floral organ observed; homozygous plants are sterile.
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N16297
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Name: gid1a-2/gid1b-3/gid1c-1 |
Price:
£11.00
|
Donor
- Yale University XingWang Deng
- Yale University Suhua Feng
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Locus
|
Stock type: individual line
|
Material type: seed
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Description
Triple mutant defective in all three homologs of the gibberellin (GA) receptor GIBBERELLIN-INSENSITIVE DWARF1 (GID1); line generated by genetic cross between two T-DNA insertional null alleles, gid1a-2 (SAIL_536_G10) and gid1c-1 (SALK_023529) and a mis-sense point mutation allele, gid1b-3, that expresses truncated protein; resistant to Basta (20mg/ml).
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Phenotype
Reduced plant size (dwarf); delayed flower formation with severe defects in floral organ development; does not respond to applied GA; seeds only germinate when the mature embryo is dissected from the seed coat.
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N16307
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Name: hapless 1 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
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Locus
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Stock type: individual line
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Material type: seed
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; strong, but not complete impact on pollen function; pollen tubes fail to leave the septum.
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N16308
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Name: hapless 2-1 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
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Locus
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Stock type: individual line
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Material type: seed
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; strong, but not complete impact on pollen function; disrupted pollen tube guidance to ovules, reduced ovule targeting by two-fold; pollen tube length is not affected in vitro or in the pistil; sperm develop normally and migrate to the pollen tube tip; sperm delivered to ovules fail to initiate fertilization.
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N16309
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Name: hapless 3 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
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Locus
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Stock type: individual line
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Material type: seed
|
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; strong, but not complete impact on pollen function; short pollen tube growth, failing to exit the style.
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N16310
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Name: hapless 4 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
|
Locus
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Stock type: individual line
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Material type: seed
|
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, affecting the male gametophyte and the female gametophyte, decreasing, but not eliminating, the function of both gametophytes; pollen tube growth is chaotic in the ovary.
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N16311
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Name: hapless 5 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
|
Locus
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Stock type: individual line
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Material type: seed
|
|
Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, affecting the male gametophyte and the female gametophyte, decreasing, but not eliminating, the function of both gametophytes; disrupted pollen grain development.
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